Petrocephalus microphthalmus

Ozouf-Costaz, Catherine, Coutanceau, Jean-Pierre, Bonillo, Céline, Belkadi, Laurent, Fermon, Yves, Agnese, Jean-François, Guidi-Rontani, Chantal & Paugy, Didier, 2015, First insights into karyotype evolution within the family Mormyridae, Cybium 39 (3), pp. 227-236 : 233

Petrocephalus microphthalmus

The karyotype is composed of 2n = 50 (2sm + 48a), NF = 52. C-bands are found in the centromeric regions of all chromosomes and many in the interstitial, pericentromeric or telomeric regions of acrocentric pairs ( Fig. 3 ). The 18S rDNA genes correspond to DAPI-negative regions. They are located in the short arms of two acrocentric pairs ( Fig. 5I, J ). The telomeric probe hybridized on all chromosomes in telomeric positions and no ITS could be recorded. Two acrocentric chromosomes bear giant telomeres in their short arms ( Fig. 5K ). The microsatellite (GAA) 10 is abundant and located in centromeric and telomeric positions ( Fig. 5L ).

Figures

Figure 3. - Karyotypes of G. petersii, GNPE, M. moorii, MAMO, I. opdenboschi, IVOP, Brienomyrus sp., BR SP, S. walkeri, STWA, and P. microphthalmus, PEMI, after conventional Giemsa staining and C-banding. Scale bar = 10 µm.

Figure 5. - Metaphase plates of Brienomyrus sp. (A, B, C, D), S. walkeri (E, F, G, H) and P. microphthalmus (I, J, K, L), showing FISH patterns of the 18S rDNA probe, indirectly DIG-labelled, detected with rhodamine; DAPI counterstaining on the same metaphase plate; patterns of the Atto 488 labelled (TTAGGG)7 probe; patterns of the Cy3 labelled (GAA)10 satellite probe. Scale bars = 10 µm.