Brunneofusispora baoshanensis X.F. Liu, Tibpromma & K.D. Hyde, 2024

Liu, Xiangfu, Tibpromma, Saowaluck, Karunarathna, Samantha C., Chethana, K. W. T., Lu, Li, Dai, Dongqin, Elgorban, Abdallah M. & Hyde, Kevin D., 2024, Morphology and multi-gene phylogeny reveal a new Brunneofusispora species from coffee in Yunnan Province, China, Phytotaxa 634 (1), pp. 16-30 : 19

Brunneofusispora baoshanensis X.F. Liu, Tibpromma & K.D. Hyde , sp. nov. ( Figure 2 )

Index Fungorum Number: IF901183; Facesoffungi Number: FoF 14924

Holotype:— ZHKU 23-0077

Etymology:— named after the location “Baoshan” where the holotype was discovered.

Saprobic on dead wood of a Coffea sp. Sexual morph: Ascomata 250–350 × 150–200 μm (x̄ = 291 × 177 μm, n = 5), solitary, gregarious, uniloculate, immersed to semi-immersed, visible as erumpent long neck on host surface, shiny, globose to subglobose, glabrous, coriaceous, brown to dark brown, rough-walled, papillate with long neck. Ostioles 130–220 × 69–120 μm, central, brown to dark brown, filled with periphyses. Peridium 13–60 μm wide, composed of 4–6 layers of brown to dark brown cells of textura angularis, the inner layer hyaline to pale brown, and heavily pigmented at the outer layer. Hamathecium comprising 1.4–4.8 μm wide, medium dense, filamentous, branched, septate pseudoparaphyses, embedded in a gelatinous matrix, longer than asci. Asci 47–93 × 10–20 μm (x̄ = 69.73 × 14.62 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindric-clavate to clavate, short pedicellate, rounded at the apex, with an ocular chamber. Ascospores 16–26 × 4–8 μm (x̄ = 20.92 × 6.21 μm, n = 60), uni to biseriate, broad fusiform to ellipsoid, mostly inequilateral, ends acute, initially hyaline, pale brown at maturity, 1-septate, constricted at the septum, with guttules, smooth-walled, with 0.9–3.3 μm mucilaginous sheath. Asexual morph: Undetermined.

Culture characteristics:— Ascospores germinated on PDA within 24 h; colonies on PDA after two months attaining a diam of 30 mm at 25 ℃, fluffy, brown with grayish-white margins, with greyish white aerial mycelia on the surface, dense, circular to irregular; reverse black, with edge greyish white.

Material examined:— China, Yunnan Province, Baoshan City, Longyang District, on a dead wood of a Coffea sp. , 30 July 2022, Li Lu, BS1-C6 (ZHKU 23-0077, holotype), ex-type living culture ZHKUCC 23-0664, ZHKUCC 23-0665.

Notes:— In the phylogenetic tree, Brunneofusispora baoshanensis formed a distinct lineage within Brunneofusispora , sister to B. sinensis , with 98% ML and 1.00 PP bootstrap support ( Figure 1 ). However, the nucleotide comparison between B. baoshanensis (ZHKUCC 23-0664, type) and B. sinensis (KUMCC 17-0030, type) showed that there are 2.15% (13/604 bp) differences in SSU, 4.66% (25/536 bp) in ITS, 1.66% (13/782 bp) in LSU, and 3.70% (35/945 bp) in TEF1-α. Morphologically, B. baoshanensis differs slightly from B. sinensis by its circular to irregular, fluffy, brown colonies with grayish-white margins, smaller ascomata (250–350 × 150–200 μm vs 325–370 μm), thicker brown to dark brown peridium (13–60 μm vs 20–45 μm), and shorter asci (47–93 × 10–20 μm vs 53–110 × 9–18 μm) ( Phookamsak et al. 2019). Therefore, B. baoshanensis is introduced as a new species based on a polyphasic approach following the guidelines of Chethana et al. (2021) and Pem et al. (2021) ( Figures 1 , 2 ). A morphological comparison between B. baoshanensis and other Brunneofusispora species is listed in Table 2.

Figures

FIGURE 1. RAxML tree based on a combined dataset of SSU, ITS, LSU, RPB2, and TEF1-α partial sequences. Bootstrap support values for maximum likelihood (ML) equal to or higher than 60% and Bayesian probability (BYPP) equal to or higher than 0.90 are mentioned above the branches (ML/BI). Newly generated sequences are shown in red. Type strains are denoted with T.

FIGURE 2. Brunneofusispora baoshanensis (ZHKU 23-0077, holotype). a, b. Ascomata on the host substrate. c. Vertical section through an ascoma. d. Ostiole. e. Peridium. f Hamathecium. g–k. Asci. l–o. Ascospores (l shows an ascospore with mucilaginous sheath). p. An ascospore in India ink. q. Germinating ascospore. r. Colony on PDA (above and below). Scale bars: b, c = 100 μm, d = 50 μm, e–k, q = 20 μm, l–p = 10 μm.