Neopestalotiopsis hydeana Huanraluek & Jayaward., 2021

Huanaluek, Naruemon, Jayawardena, Ruvishika S., Maharachchikum- Bura, Sajeewa S. N. & Harishchandra, Dulanjalee L., 2021, Additions to pestalotioid fungi in Thailand: Neopestalotiopsis hydeana sp. nov. and Pestalotiopsis hydei sp. nov., Phytotaxa 479 (1), pp. 23-43 : 33

Neopestalotiopsis hydeana Huanraluek & Jayaward. sp. nov. ( Fig. 3 )

Index Fungorum number: IF558099; Facesoffungi number: FoF 09459

Etymology:— In honour of Assoc. Prof. Kevin D. Hyde.

Associated with leafspots and fruit rots. Conidiomata pycnidial in culture on PDA at 25 °C after 12 days, globose to oval, solitary or aggregated in clusters, semi-immersed, black, 385–2,000 μm diam.; exuding globose, black, glistening, conidial masses. Conidiophores indistinct, often reduced to conidiogenous cells. Conidiogenous cells discrete, fusiform, hyaline, cylindrical to subcylindrical or ampulliform to lageniform, rugose-walled, simple, 8–14 × 2–4 μm. Conidia fusoid, ellipsoid, straight to slightly curved, 4-septate, 10–25 × 6–8 µm; basal cell conic to acute with a truncate base, hyaline, thin-walled, 3–5 μm long; three median cells doliiform, 13–16 μm long, wall versicolorus, septa darker than rest of the cell (second cell from base pale brown, 3–5 μm long; third cell darker brown, 3–5 μm long; fourth cell darker brown, 4–6 μm long); apical cell 2–6 μm long, hyaline, cylindrical to subcylindrical, rugose, thin-walled; with 2–4 tubular, apical appendages, arising as an extension of the apical cell, unbranched, attenuated, flexuous, 4–23 μm long; basal appendage single, tubular, unbranched, centric, 3–6 μm long.

Material examined:— THAILAND. Khun Chae, Wiang Pa Pao District, Chiang Rai, on leaf of Artocarpus heterophyllus (Moraceae) , 3 September 2018, Naruemon Huanraluek, CKKA (holotype MFLU 20–0471), (extype MFLUCC 20–0132); Chiang Rai, on fruit of Annona squamosa (Annonaceae) , 21 August 2018, Kushan C. Mallikarathne,An01 ( MFLU 20–0478, MFLUCC 20–0130); Chiang Rai, on fruit of Garcinia mangostana (Clusiaceae) , 21 August 2018, Kushan C. Mallikarathne, Mg02–2 ( MFLU 20-0476, MFLUCC 20–0128); Chiang Rai, on fruit of Vitis vinifera (Vitaceae) , 21 August 2018, Kushan C. Mallikarathne, Go1 ( MFLU 20–0477, MFLUCC 20–0127); Chiang Rai, on fruit of Garcinia mangostana (Clusiaceae) , 21 August 2018, Kushan C. Mallikarathne, Mg02–3 ( MFLU 20–0479, MFLUCC 20–0129); Chiang Rai, on fruit of Garcinia mangostana (Clusiaceae) , 21 August 2018, Kushan C. Mallikarathne, Mg02–1 ( MFLU 20–0475, MFLUCC 20–0126); Chiang Rai, on leaf of pteridophyte 1 November 2018, Ruvishika S. Jayawardena and Naruemon Huanraluek, BG 012 ( MFLU 20–0472, MFLUCC 20– 0136); Chiang Rai, on leaf of Alpinia malaccensis (Zingiberaceae) , 9 October 2018, Ruvishika S. Jayawardena and Naruemon Huanraluek, BG 002–1 ( MFLU 20–0473, MFLUCC 20–0133); Chiang Rai, on fruit of Citrus sp. (Rutaceae) , 21 August 2018, Kushan C. Mallikarathne, ciol ( MFLU 20–0480, MFLUCC 20–0131); Chiang Rai, on leaf of Alpinia galangal (Zingiberaceae) , 9 October 2018, Ruvishika S. Jayawardena and Naruemon Huanraluek BG 007–1 C ( MFLU 20–0474, MFLUCC 20–0134).

Notes:— In the phylogenetic tree Neopestalotiopsis hydeana formed a sister clade to N. musae MFLUCC 15– 0776 and N. alpapicalis MFLUCC 17–2545, but they differ in morphology. Neopestalotiopsis hydeana has smaller conidia than N. musae (18.6–25 × 4.1–5 μm) ( Table 2) ( Hyde et al. 2016), but its conidia are bigger than those of N. alpapicalis (14–22.3 × 5–6.8 μm) and it has longer apical appendages than N. alpapicalis (5.6–15 μm long) ( Kumar et al. 2019). There are 8bp, 12bp (and a deletion of 27bp in the front) and 20 bp differences between N. hydeana and N. musae in ITS, TUB 2 and Tef1, respectively. There are 4bp, 21bp and 29bp differences between N. hydeana and N. alpapicalis in ITS, TUB 2 and Tef1, respectively. Based on both morphology and phylogenetic evidence we introduce this as a new species.

Figures

FIGURE 3. Neopestalotiopsis hydeana. a. Colony top view. b. Colony reverse view. c. Conidiomata on PDA. d–f. Conidiogenous cells. g–k. Conidia. Scale bars d = 20 µm, e–g = 10 µm, h–k = 10 µm.