Rickettsiales

Su, Si, Hong, Mei, Cui, Meng-Yu, Gui, Zheng, Ma, Shi-Fa, Wu, Lin, Xing, Li-Li, Mu, Lan, Yu, Jing-Feng, Fu, Shao-Yin, Gao, Rui-Juan & Qi, Dong-Dong, 2023, Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac 37 b) in Inner Mongolia, China, Parasite (Paris, France) 30 (58), pp. 1-13 : 5

Phylogenetic analysis of Rickettsiales bacterium Ac37b

The Rickettsiales bacterium Ac37b (OP286853 and

OP286855) sequences were randomly selected to build a phylogenetic tree, named after regions and displayed on the phylogenetic tree with squares and triangles ( Fig. 9a ). The phylogenetic tree of the 16S rRNA gene showed that Rickettsiales bacterium Ac37b found in Inner Mongolia is in the same branch as the Rickettsiales bacterium Ac37b (CP009217) found in Amblyomma cajennense from Brazil, and their homology is 100% according to sequence alignment analysis using MEGA 7.0 software.

Figures

Figure 9. Phylogenetic tree of Rickettsiales bacterium Ac37b, Rickettsia bellii, Rickettsia raoultii, Anaplasma and Coxiella in ticks based on neighbor-joining (NJ) modeling; only values higher than 60 were added to the tree branches. (a) Phylogenetic tree of Rickettsiales bacterium Ac37b identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1320 bp) and triangles (1430 bp). (b) Phylogenetic tree of Rickettsia bellii identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1109 bp). (c) Phylogenetic tree of Rickettsia raoultii identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (855 bp). (d) Phylogenetic tree of Anaplasma identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1455 bp) and triangles (547 bp). (e) Phylogenetic tree of Coxiella identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1463 bp).