Anaplasma, Theiler, 1910

Su, Si, Hong, Mei, Cui, Meng-Yu, Gui, Zheng, Ma, Shi-Fa, Wu, Lin, Xing, Li-Li, Mu, Lan, Yu, Jing-Feng, Fu, Shao-Yin, Gao, Rui-Juan & Qi, Dong-Dong, 2023, Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac 37 b) in Inner Mongolia, China, Parasite (Paris, France) 30 (58), pp. 1-13 : 7

Phylogenetic analysis of Anaplasma

The Anaplasma (OP286854 and OP286856) sequences were randomly selected to build a phylogenetic tree, named after regions and displayed on the phylogenetic tree with squares and triangles ( Fig. 9d ). The phylogenetic tree of the 16S rRNA gene shows that Anaplasma found in Inner Mongolia is on the same branch as Anaplasma ovis strain Ao89 ( KJ 639879). The sequences were compared and analyzed using MEGA 7.0 software. They show that the 16S rRNA gene sequence of Anaplasma in Inner Mongolia has 99.93% homology with A. ovis strain Ao89 ( KJ 639879) found in the Qilian Mountains, Lanzhou.

Figures

Figure 9. Phylogenetic tree of Rickettsiales bacterium Ac37b, Rickettsia bellii, Rickettsia raoultii, Anaplasma and Coxiella in ticks based on neighbor-joining (NJ) modeling; only values higher than 60 were added to the tree branches. (a) Phylogenetic tree of Rickettsiales bacterium Ac37b identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1320 bp) and triangles (1430 bp). (b) Phylogenetic tree of Rickettsia bellii identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1109 bp). (c) Phylogenetic tree of Rickettsia raoultii identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (855 bp). (d) Phylogenetic tree of Anaplasma identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1455 bp) and triangles (547 bp). (e) Phylogenetic tree of Coxiella identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1463 bp).