Pontogammarus robustoides

Brakovska, Aija & Paidere, Jana, 2019, Protocol Optimization For Genomic Dna Extraction And Rapd-Pcr Of Alien Ponto-Caspian Amphipod Pontogammarus Robustoides, Acta Biologica Universitatis Daugavpiliensis 19 (2), pp. 209-217 : 210

Pontogammarus robustoides collection and processing

Samples of Pontogammarus robustoides from the Daugava water reservoirs in Latvia were collected by qualitative sampling in May – September from 2014 to 2018 ( Fig. 1 ). The collected water sample material was preserved immediately after collecting by adding 98% ethanol to water sample hence the final concentration in the sample is ± 70%. After the splitting collected material into the species, the species resulting material was stored in 98% ethanol. The preservation and storage of Pontogammarus robustoides samples in 98% ethanol was cost-effective and appropriate for many molecular analyses ( Harris et al. 2005).

Figures

Fig.1. Localities of sampling sities in the Latvian reservoirs.