Absidia pernambucoensis D.X. Lima, C.M. Souza-Motta

Lima, Diogo X., Cordeiro, Thalline R. L., De Souza, Carlos A. F., De Oliveira, Rafael J. V., Lee, Hyang B., Souza-Motta, Cristina M. & De Azevedo Santiago, André L. C. M., 2020, Morphological and molecular evidence for two new species of Absidia from Neotropic soil, Phytotaxa 446 (1), pp. 61-71 : 63-66

Absidia pernambucoensis D.X. Lima, C.M. Souza-Motta & A.L. Santiago, sp. nov. FIG. 4

Mycobank number: MB 833217

Etymology— pernambucoensis . Referring to the state (Pernambuco) where the species was first isolated.

Colony initially white and becoming dark gray (MP 16B6), covering the Petri dish (9 cm diam.) and reaching the plate lid (1.5 cm alt.) in some areas after five days at 25°C on MEA; reverse cream-colored (MP 12H3). Odorless. Sporangiophores (65–) 85–220 (–240) × 2.5–5 µm, erect, smooth-walled and hyaline, growing along the stolons and terminally, solitary or usually in whorls of 2–5 (–8), often with a septum below the sporangium. Stolons smooth-walled and hyaline, up to 4–15 µm diam. Rhizoids hyaline, poorly branched, growing in one direction like a broom. Sporangia pyriform to subglobose, smooth-walled, 20–35 µm diam., apophysate, with a bell-shaped apophysis; columellae mostly subglobose, some hemispherical, 10–25 µm diam., smooth-walled with one or occasionally two projections on its central part; collar sometimes present.A solitary projection is usually present on subglobose columellae, 3.5–6.5 × 1.2– 2.5 µm, and a double projection is usually present in hemispherical columellae, up to 5 × 1.5–2 µm. Sporangiospores are regular in size and shape, mostly cylindrical and some slightly cuneiform, 3.5–5 × 1.5–2.5 µm. Chlamydospores are absent. Zygospores were not observed.

Habitat: soil

Distribution: Pernambuco, Brazil.

Specimen examined: BRAZIL, Pernambuco, Tamandaré (8°43′09′′S, 35°10′37′′W), soil., 2018, D.X. Lima (URM 93638 – holotype, ex-type culture – URM 7219).

Media and temperature: On MEA: at 15°C—slow growth (4.7 cm in 192 hours); at 20°C—good growth (9 cm in 120 hours) and good sporulation; at 25°C—good growth with excellent sporulation (9 cm in 120 hours); at 30°C—slower growth than at 25°C (9 cm in 168 hours) and good sporulation; at 35°C—lack of growth. The growth of A. pernambucoensis on PDA was slower than on MEA, with low sporulation at all temperatures.

Figures

FIGURE 4. Absidia pernambucoensis (holotype URM 93638). a. Branched sporangiophore. b-c. Unbranched sporangiophore with a columella and a single projection. d. Unbranched sporangiophore with a columella and double projection. e. Unbranched sporangiophore with a columella without projection. f. Unbranched sporangiophores on a stolon. g. Cylindrical and cuneiform sporangiospores.