Stropharia atroferruginea Khan M.B., Fiaz & Khalid, 2019

Khan, Muhammad Binyamin, Ishaq, Muhammad, Kiran, Munazza, Fiaz, Muhammad & Khalid, Abdul Nasir, 2019, Stropharia atroferruginea (Agaricales, Strophariaceae), a new species from Battagram district, Pakistan, Phytotaxa 409 (2), pp. 83-92 : 88

Stropharia atroferruginea Khan M.B., Fiaz & Khalid , sp.nov., ( Figure 2 A & B )

Etymology:—From Latin,“ atro ”meaning dark and “ ferruginea ”meaning iron-rusty color referring to the distinct dark reddish brown pileus color.

MycoBank number: MB829196

Holotype:— PAKISTAN, Khyber Pakhtunkhwa, Battagram district. Muhammad Binyamin Khan, voucher number HU32916 ! GenBank accession numbers MK141061 , MK434190 , MK446222 , and MK434326 ( ITS), MK344168 and MK433557 ( LSU).

Diagnosis:—Pileus dark red to dark reddish brown with gray, round squamules; lamellae decurrent, gray becoming black with maturity; annulus large, distinct and cogwheeled; spores ellipsoid to oblong.

Distribution:—Currently known only from Battagram district, Khyber Pakhtunkhwa, Pakistan, in forests of Pinus roxburghii mixed with Quercus incana .

Additional materials examined:— PAKISTAN. Khyber Pakhtunkhwa, Battagram district, forest of Pinus roxburghii , mixed with Quercus incana Roxb. 17 July 2017, Muhammad Binyamin Khan, Isotype HU32915, Genbank accession number MK141061, MK434190, MK446222, and MK434326 (ITS), MK344168 and MK433557 (LSU).

Description:—Pileus 2–5.2 cm broad, firstly convex, becoming flattened then depressed, dark red (2.5 Y 3/6) to dark reddish brown (5YR3/3–3/4) becoming more gray squamules; margin slightly incurved,crenate, with appendiculate velar remnants. Lamellae adnexed with decurrent teeth, gray (2.5 Y 5/1–6/1), becoming black (7.5YR 2.5/1) with maturity; margin whitish, irregular. Stipe 2–3.9 cm broad, central, clavate or with a sub-bulbous base, white (5Y 8/1) at the apex but yellowish brown (10YR 5/4–5/8) towards the base. Stipe context glabrous, white gray, solid, ring are present and volva are absent. Annulus large and distinct, cogwheeled, yellowish on the upper stipe and with whitish appendiculate velar remnants. glabrous, white gray stipe surface.Volva absent. Basidiospores [n/b/p] [100/5/5](8.4) 9.4–12.1 (13.7) × (5.3) 6.3–8.1 (9.7) μm,Q = (1.2) 1.4–1.7 (2.1); Qe = 1.5 μm, ellipsoid to oblong, angular, smooth and thick-walled, smooth with central germ pore brownish in 5%KOH and bluish black to light brownish in Congo red. Basidia (9.9) 15.7–23.9 (25.4) × (6.2) 6.8–9.4 (9.6) μm, clavate, thin-walled, 2–4 spored. Pleurocystidia (23.4) 29.7–34.4(34.8) × (6.4) 6.8–8.2(8.5) μm, clavate, with a capitate apex. Cheilocystidia (21) 21.3–30.1(32.6) × (5.6) 6.3–8.7(9.3) μm, clavate, with a capitate apex, without oil content. Caulocystidia (52.3) 55.7–62.1 (62.8) × (3.8) 4.9–6.2 (6.3) μm, smooth and thin-walled. Pileipellis anintricate trichoderm, 2.8–3.8 μm thick,with branched hyphae; clamp-connections present. Stipitipellis a cutis, 7.1–9.6 μm thick, composed of regularly unbranched, septate hyphae; clamp-connections present. Partial veil at stipe base comprised of loosely interwoven, undifferentiated hyphae, which are 8.7–15.7μmdiam. Gill trama regular, inflate and smooth with thick-walled hyphae, 16–24 μm in diameter.

Figures

FIGURE 2. Maximum-Likelihood phylogenetic tree generated from the ITS dataset. ML bootstrap values are shown above the nodes. Stropharia atroferruginea is shown in blue.