Scleroramularia musae G.Y. Sun & L. Gao, 2015

Gao, Liu, Wei, Zhuoya, Wang, Bo, Gleason, Mark L., Sun, Guangyu & Zhang, Rong, 2015, A new species of Scleroramularia associated with sooty blotch and flyspeck in Southern China, Phytotaxa 226 (3), pp. 261-268 : 265-267

Scleroramularia musae G.Y. Sun & L. Gao , sp. nov. ( Fig. 2 )

MycoBank MB 812578

Type:— CHINA. Guangdong: Zhanjiang, October 2011, L. Gao, W. H. Li & H. C. Chen, GLZJXJ13 B (holotype HMAS 246378! ex-type culture CGMCC 3.17647!)

Etymology:—Named after the host from which it was collected.

On SNA. Mycelium creeping, superficial and submerged, consisting of hyaline, smooth, branched, septate, 1.5–2 μm diam hyphae. Conidiophores mostly reduced to conidiogenous cells, or with one supporting cell. Conidiogenous cells solitary, erect, intercalary on hyphae, subcylindrical, straight, with 1–2 terminal loci, rarely with a lateral locus, 1.5–9 × 1.5–2.5 μm; scars thickened, darkened and somewhat refractive, 0.5–1 μm wide. Conidia in branched chains, hyaline, smooth, finely guttulate, straight or gently curved if long and thin; basal conidia mostly narrowly cylindrical, 0–4-septate, 20–80 × 1.5–3 μm; intercalary and terminal conidia narrowly cylindrical, subcylindrical, narrowly ellipsoid to fusoid-ellipsoid, 0–7-septate, (10–)12–39 × (2–)2.5(–3) μm; hila thickened and somewhat darkened, 0.5–1 μm wide.

Culture characteristics:—Colonies after 2 weeks at 25 ° C on SNA spreading with sparse aerial mycelium and somewhat feathery margin, reaching 14 mm diam, with white surface. On PDA spreading, flattened, with sparse aerial mycelium, surface folded, white and producing red brown pigment; reverse with patches of iron-grey in middle, orange in outer region, reaching 18 mm diam after 2 weeks at 25 ° C. It produced sclerotia on PDA after 7 weeks. On MEA colonies were slower growing, and had sparse aerial mycelium; surface white with raised center; reverse olivaceous gray in center and canary yellow in outer region, reaching 14 mm diam. On OA flattened, spreading, with sparse aerial mycelium, and even, raised margins, white, reaching 17 mm diam; reverse umber in centre and canary yellow in outer region.

Substratum:—On the surface of Japanese banana ( Musa basjoo ) fruit

Geographical distribution:—Known only from Zhanjiang city, Guangdong Province, China.

Comments:—Morphologically, conidia of S. musae have more septa than other known species, whether basal or intercalary or terminal conidia. For basal conidia, S. musae has as many as 4 septa per conidium whereas other species in this genus have only 3 or fewer septa ( Li et al. 2011). Intercalary and terminal conidia of S. musae possessed up to 7 septa compared to 3 septa for other known species in the genus.

Figures

FIGURE 2. The single most parsimonious trees obtained from a heuristic search combined ITS and TEF sequence alignment. Numbers at branching nodes represent bootstrap values>50 % (1000 replicates). The tree was rooted to Beauveria bassiana (GenBank AY532027 and AY531936 for ITS and TEF, respectively).