Toxoplasma gondii detection

Meister, Seraina L., Wenker, Christian, Wyss, Fabia, Zühlke, Irene, In, Veiga, es Berenguer & Basso, Walter U., 2022, Syngamus trachea in free-ranging white stork (Ciconia ciconia) nestlings in Switzerland, International Journal for Parasitology: Parasites and Wildlife 18, pp. 76-81 : 77

2.5.2. Internal organs for Toxoplasma gondii detection

DNA from the white storks’ internal organs (brain, lung, heart, liver, spleen) was isolated using the Spin-Column Protocol for animal tissues from the DNeasy® Blood & Tissue Kit (QIAGEN GmbH, Hilden, Germany) with the following modification ( Berger-Schoch et al., 2011): 500 μg tissue were mixed with 100 μl proteinase K and 900 μl ATL buffer, followed by an over-night incubation at 56 ◦ C; 200 μl of the digested material were then further processed according to the manufacturer’ s instructions.

2.6. PCR