Podospora anserina, (Rabenh.) Niessl (Rabenh.) Niessl

Ferrari, Roselyne, Gautier, Valérie, Tangthirasunun, Narumon, Tong, Laetitia Chan Ho & Silar, Philippe, 2024, Characterization of the Podospora anserina (Rabenh.) Niessl peroxidase gene family, Cryptogamie, Mycologie 20 (2), pp. 11-28 : 20

EXPRESSION OF PEROXIDASE GENES IN P. ANSERINA MYCELIUM

We evaluated the expression of peroxidase genes during two days and four days of growth on M2 medium ( Table 1 ), by analyzing microarrays and RNAseq data generated in previous studies ( Bidard et al. 2012; Silar et al. 2019) ( Table 1 ). We could evidence expression of all peroxidase genes (except for the “ Pa_2_10 ” pseudogene). The hybrid B peroxidase Pa_2_9370 and the aromatic peroxygenase Pa_0_370 genes were highly transcribed; on the contrary, the class II peroxidase Pa_1_5970 and the aromatic peroxygenase Pa_5_12205 gene were poorly expressed, a feature compatible with the lack of the aromatic peroxygenase Pa_5_ 1220 in secretomes data. Note that analysis of RNAseq data from perithecia showed that the class II peroxidase Pa_1_5970 gene was much more transcribed in these sexual fruiting bodies than in the mycelium, especially in four-day-old perithecia.