Jackrogersella minutella (Sydow. & P. Sydow) L. Wendt, Kuhnert & M. Stadler

Karimi, Omid, Thilini Chethana, K. W., Farias, Antonio R. G. & Li, Qirui, 2023, Two new records of Xylariales species from Northern Thailand, Phytotaxa 598 (3), pp. 187-205 : 198

Jackrogersella minutella (Sydow. & P. Sydow) L. Wendt, Kuhnert & M. Stadler , Mycological Progress 17: 27 (2018)

( FIGURES 2 and 4 )

Index Fungorum number: IF 819749, Facesoffungi number: FoF 14032

Saprobic on Quercus kingiana .

Sexual morph: Stromata 5–10 × 2–4 mm (x̄ = 6.5 × 2.8 mm, n = 15), pulvinate to peltate, often constricted at base, with inconspicuous to conspicuous perithecial mounds, surface brown to dark brown, KOH-extractable vinaceous to rusty pigments. Perithecia 0.6–0.8 mm high, 0.3–0.7 mm broad (x̄ = 0.7 × 0.5 mm, n = 50), subspherical to obovate. Ostioles 0.1–0.2 mm diam., papillate not encircled by a disc, stromata peltate, pulvinate to peltate, or effused pulvinate. Asci 102–216 µm total length (x̄ = 148 µm, n = 50), spore-bearing parts 44–59 × 2–4.6 µm (x̄ = 53.5 × 3.5 μm, n = 50), 8-spored, cylindrical, with tubular, apical apparatus bluing in Melzer’s reagent, 1.5–2.4 µm high × 1.1–2 µm broad (x̄ = 2 × 1.5 µm, n = 50) with a slightly flared apex. Ascospores 4.1–7 × 2–4.2 µm (x̄ = 5.8 × 3.3 µm, n = 50), ellipsoid-inequilateral, conspicuous, straight, dark olive-brown to dark brown, smooth, biguttulate, with narrowly rounded ends. Asexual morph: Undetermined.

Material examined: Thailand, Chiang Mai Province, Chomthong district, Doi Inthanon National Park , Kew Mae Pan nature trail, on decaying barks of Quercus kingiana ( Fagaceae ), 18 October 2021, MC Samarakoon DI005 ( MFLU 23-0051 , new host and geographical record) .

GenBank number: MFLU 23-0051: ITS = OP 077351, LSU = OQ552823, rpb2 = OQ597843, tub2 = OQ601534.

Known distribution: India ( Chuzho & Dkhar 2019), Portugal (Kuhner et al. 2016), Philippines ( Sydow & Sydow 1913), Norway ( Norden 2014), Iran ( Raei et al. 2012), Spain (Stadler 2004), France ( Rogers & Candoussau 1980), Britain ( Anderson 2009), Thailand (this study).

Known hosts: Quercus sp. ( Chuzho & Dkhar 2019), Quercus sp. ( Norden 2014), Caprinus betulus ( Raei et al. 2012) , Quercus kingiana (this study).

Notes – Our isolate morphologically resembles J. minutella in having pulvinate to peltate stromata with a brown surface, often constricted at the base, the KOH-extractable vinaceous to rusty pigments, and ellipsoid-inequilateral, dark olive-brown to dark brown, smooth, biguttulate ascospores ( Hsieh & Rogers 2005, Kuhner et al. 2016). Our phylogenetic analysis based on combined ITS, LSU, rpb2, and tub2 data revealed that our strain clusters with J. minutella ( CBS 119015) with 100% maximum likelihood support and 1.00 Bayesian posterior probability and all the characters of our specimen identical to the J. minutella ( CBS 119015). The current study presents the first known record of J. minutella from Thailand.

Figures

FIGURE 2. Phylogram generated from maximum likelihood analysis based on combined ITS, LSU, rpb2, and tub2 sequence data of Hypoxylaceae taxa. Maximum likelihood bootstrap values greater than or equal to 60% and Bayesian posterior probabilities greater than 0.95 are given near nodes, respectively. The tree was rooted with Xylaria hypoxylon (CBS 122620) and Xylaria polymorpha (MUCL 49884). The newly generated sequence is indicated in blue.All the type specimens are in bold.

FIGURE 4. Jackrogersella minutella (MFLU23-0051, new host and geographical record). a, b Stromata. c, d Sections of an ascoma. e KOH-extractable pigments. f–h Asci. i Paraphyses. j Apical ring stained in Mezler reagent. k Perispore. i–o Ascospores. Scale bars: b, c = 1 mm, d = 500 µm, e = 300 µm, f–i =20 µm, j–o = 5 µm.