Arthrocatena antalyensis (Sert & Sterfl.) Piątek, Stryjak-Bogacka & Czachura 2024

Piątek, Marcin, Stryjak-Bogacka, Monika & Czachura, Paweł, 2024, Arthrocatenales, a new order of extremophilic fungi in the Dothideomycetes, MycoKeys 108, pp. 47-74 : 47-74

Arthrocatena antalyensis (Sert & Sterfl.) Piątek, Stryjak-Bogacka & Czachura comb. nov.

Figs 2 , 3 , 4

Basionym.

Capnobotryella antalyensis Sert & Sterfl. , Mycol. Res. 111 (10): 1237 (2007).

Typus.

Turkey, Antalya , isolated from the surface of a child’s grave in Side museum (holotype: ACBR MA 4659 ) .

DNA barcodes (from analysed strains).

ITS ( OR 096278, OR 096279), LSU ( OR 096282, OR 096283), SSU ( OR 096280, OR 096281), rpb 2 ( OR 096699, OR 096700).

Description.

Mycelium composed of branched, septate, pale brown or brown, smooth, straight, flexuose or torulose, thin-walled hyphae, 3.5–7.0 µm wide, consisting of elongated, subglobose, broadly ellipsoidal or pyriform cells, sometimes anastomosing; hyphae develop into arthroconidia, chlamydospore-like cells or chlamydospores. Arthroconidia ellipsoid or broadly ellipsoid, rarely barrel-shaped, brown, smooth, one-septate, 9.0–19.0 (– 23.0) × 6.5–8.5 µm, produced intercalary or rarely on side branches, single or in chains. Chlamydospore-like cells spherical, brown, smooth, aseptate, 7.0–12.0 × 7.0–10.0 µm, produced intercalary, in simple or branched chains. Chlamydospores spherical, brown, smooth, muriformly septate, 12.5–15.0 × 11.0–14.0 µm, produced intercalary, single between chlamydospore-like cells.

Culture characteristics.

Colonies on MEA erumpent, spreading, with elevated and folded center, greenish olivaceous, forming concentric rings, reaching 8 mm diam after 4 weeks growth at 15 ° C and 12 mm diam after 4 weeks growth at 25 ° C, surface with moderate aerial mycelium, margin smooth and entire, darker than the remaining part. Reverse black. Colonies on PDA erumpent, spreading, with elevated and folded center, greenish olivaceous, forming indistinct concentric rings, reaching 10 mm diam after 4 weeks growth at 15 ° C and 14 mm diam after 4 weeks growth at 25 ° C, surface with sparse aerial mycelium, margin smooth and undulate, concolours with the remaining part. Reverse black.

Specimens examined.

Poland, Silesian Province, Katowice County: Katowice-Bogucice, municipal greenery, isolated from sooty mould community on Tilia cordata leaves, 10 Sept. 2018, leg. M. Piątek, W. Bartoszek & P. Czachura ( KRAM F - 59837; culture: G 57 = CBS 150720); Podkarpackie Province, Rzeszów County: Rzeszów – Generała Władysława Andersa, municipal greenery, isolated from sooty mould community on Pinus nigra needles, 17 Sept. 2018, leg. M. Piątek, W. Bartoszek & P. Czachura ( KRAM F - 59838; culture: G 385 = CBS 150721).

Notes.

Arthrocatena antalyensis differs from Arthrocatena tenebrosa in having larger arthroconidia (6.0–11.5 × 3.0–5.5 μm in A. tenebrosa ; Egidi et al. 2014) and formation of chlamydospore-like cells and muriformly septate chlamydospores.

Figures

Figure 2. Morphology of Arthrocatena antalyensis (strain CBS 150720, e – h slide culture on PDA): a, b general view and detailed view of upper side of colony on MEA after 4 weeks of growth at 25 ° C c, d general view and detailed view of upper side of colony on PDA after 4 weeks of growth at 25 ° C e general view of hyphae f, g straight hyphae (note anastomosing hyphae visible on figure g) h terminal, flexuose and torulose hyphae. Scale bars: 50 µm (e); 10 µm (f – h).

Figure 3. Morphology of Arthrocatena antalyensis (strain CBS 150720, slide culture on PDA): a general view of hyphae, arthroconidia and chlamydospore b – d arthroconidia e chlamydospore-like cells f – g chlamydospore-like cells and muriformly septate chlamydospores (indicated by arrows). Scale bars: 50 µm (a); 10 µm (b – g).

Figure 4. Morphology of Arthrocatena antalyensis (strain CBS 150720, on MEA): a, b arthroconidia, arrows indicate disintegrating chains of arthroconidia c – e disintegrated arthroconidia. Scale bars: 10 µm (a – e).