taxonID	type	description	language	source
03F62E65F756E822FF78F9EBFB8F4B4F.taxon	etymology	Etymology: — The species epithet ‘ camelliae-sinensis ’ refers to the host from which the fungus was originally isolated.	en	Chi, Mei-Feng, Liang, Xiang-Dong, Wu, Na, Dissanayake, Asha J., Liu, Jian-Kui (2025): Helminthosporium camelliae-sinensis sp. nov., a hyphomycete associated with dead twigs of Camellia sinensis in Sichuan, China. Phytotaxa 716 (3): 189-202, DOI: 10.11646/phytotaxa.716.3.3, URL: https://doi.org/10.11646/phytotaxa.716.3.3
03F62E65F756E822FF78F9EBFB8F4B4F.taxon	materials_examined	Holotype: — HAKS 144246 Saprobic on dead twigs of tea (Camellia sinensis L.). Sexual morph: Undetermined. Asexual morph: Colony on natural substrate superficial, effuse, hairy, dark brown to black, glistening. Mycelium superficial, numerous, hairy, black, scattered, solitary or fasciculate, septate, unbranched, smoothed, thick‐walled hyphae. Conidiophores 269 – 842 µm long (x = 541 µm, n = 20), 15 – 23 µm wide (x = 18 µm, n = 20), macronematous, mononematous, erect, simple, smooth, thick‐walled, subcylindrical, straight to slightly curved, septate, dark brown to black, obtuse at apex. Conidiogenous cells mono- to poly‐tretic, integrated, terminal and intercalary, cylindrical, brown, smooth. Conidial secession schizolytic. Conidia 33 – 77 µm long (x = 48 µm, n = 40), 8 – 15 µm wide (x = 11 µm, n = 40), tapering to 3.8 – 8.6 µm (x = 6.2 µm, n = 20) at the distal end, with a blackish-brown 3 – 5 µm (x = 4 µm, n = 40) wide scar at the base, 5 - 8 distoseptate; with an angular lumina, wall up to 2.3 – 3.5 µm (x = 2.8 µm, n = 20) thick, cylindrical, obclavate, phragmoconidia, acropleurogenous, straight or curved, uneven width, rounded at apex, thick-walled, light green brown to medium brown. Culture characteristics: — Conidia germinating on PDA within 24 h and germ tubes arising from cells of the arms. Colonies growing on PDA reached a diam. of 21 mm after two weeks at 25 ° C. Flower-shaped, anomalistic, white-edged, internally pale green and obvious circles, reverse almost yellowish-brown with pale yellow edges. Material examined: — CHINA, Sichuan Province, Qionglai City, Tiantaishan Town, 30 ° 15 ′ 58 '' N, 103 ° 6 ′ 25 '' E, 1,000 – 1,250 m, on dead tea twigs (Camellia sinensis), 18 September 2023, M. F. Chi, X. D. Liang T 24 - 1 (HKAS 144246, holotype; ex-type living culture, CGMCC 3.27822); ibid., T 24 - 2 (HUEST 24.0218, paratype; living culture, UESTCC 24.0201). Notes: — Phylogenetic analysis of the combined dataset, including ITS, LSU, SSU, rpb 2 and tef 1 - α loci, revealed that our collection forms a distinct clade closely related to Helminthosporium yunnanense (HJAUP C 2071) and H. austriacum (CBS 139924, CBS 142388) (Fig. 1). In the NCBI BLASTn search, the closest match to our isolate (UESTCC 24.0201) was H. austriacum (CBS 139924). A comparison of ITS, LSU, SSU, rpb 2, and tef 1 - α sequences from our collection with the type strain of H. austriacum (CBS 139924) showed 95.95 % similarity (474 / 494 bp, 1 gap), 99.41 % similarity (842 / 847, 2 gaps), 99.90 % similarity (1,024 / 1,025, 1 gap), 97.85 % similarity (1,002 / 1,024, without gaps), and 97.61 % similarity (735 / 753, without gaps), respectively. Morphologically, the conidiophores of our collection (269 – 842 × 15 – 23 µm) are shorter and wider than those of H. austriacum (275 – 700 (– 920) × 11.5 – 19 µm) (Voglmayr et al. 2017), and longer and wider than those of H. yunnanense (560 – 680 × 12.5 – 15.5 µm) (Liu et al. 2022). Our collection has 5 - 8 distoseptate conidia, which are more than those of H. yunnanense (4 - 7 distoseptate) but fewer than H. austriacum (4 - 10 distoseptate). While the conidia of both H. austriacum and H. yunnanense are pale brown, the conidia of our collection exhibit a slightly greenish-brown color (Hu et al. 2023). The shape of the conidia of our collection is obclavate and lunate, differing from the obpyriform to lageniform shape of H. austriacum and the obclavate, sigmoid shape of H. yunnanense. Additionally, our collection's conidia are wider (15 – 23 µm) compared to H. yunnanense (9 – 11 µm) and H. austriacum (10.0 – 19.8 µm) (Hu et al. 2023). Thus, based on these morphological observations and phylogenetic evidence, our collection is described as a new species.	en	Chi, Mei-Feng, Liang, Xiang-Dong, Wu, Na, Dissanayake, Asha J., Liu, Jian-Kui (2025): Helminthosporium camelliae-sinensis sp. nov., a hyphomycete associated with dead twigs of Camellia sinensis in Sichuan, China. Phytotaxa 716 (3): 189-202, DOI: 10.11646/phytotaxa.716.3.3, URL: https://doi.org/10.11646/phytotaxa.716.3.3
03F62E65F754E82CFF78FA95FAFC4D76.taxon	description	Saprobic on dead twigs of Camellia sinensis. Sexual morph: Undetermined. Asexual morph: Colony on natural substrate superficial, effuse, hairy, dark brown to black. Mycelium superficial, numerous, hairy, black, scattered, solitary or fasciculate, septate, unbranched, smooth, thick‐walled hyphae. Conidiophores 402 – 723 µm long (x = 660 µm, n = 20), 12 – 24 µm wide (x = 21 µm, n = 20), macronematous, mononematous, erect, straight or flexuous, solitary or in groups of 2 - 4, thick‐walled, subcylindrical, septate, brown to black, obtuse at apex. Conidiogenous cells monoto poly‐tretic, integrated, terminal and intercalary, cylindrical, brown, smooth. Conidial secession schizolytic. Conidia 45 – 96 µm long (x = 65 µm, n = 40), 16 – 29 µm wide (x = 21 µm, n = 40), tapering to 5.8 – 10.2 µm (x = 7.7 µm, n = 20) at the distal end, with a blackish-brown 4 – 7 µm (x = 5.5 µm, n = 40) wide scar at the base, 6 – 12 distoseptate; with an angular lumina, wall up to 3.6 – 4.9 µm (x = 4.3 µm, n = 20) thick. cylindrical, obclavate, phragmoconidia, acropleurogenous, verruculose, straight or flexuous, uneven width, rounded at apex, thick-walled, pale taupe brown to dark brown. Culture characteristics: — Conidia germinating on PDA within 24 h and germ tubes arising from cells of the arms. Colonies growing on PDA reached a diam. of 22 mm after 2 weeks at 25 ° C. Velvety, white to pale green, raised in center, with denser mycelium at the center; reverse dark brown to black with white edges.	en	Chi, Mei-Feng, Liang, Xiang-Dong, Wu, Na, Dissanayake, Asha J., Liu, Jian-Kui (2025): Helminthosporium camelliae-sinensis sp. nov., a hyphomycete associated with dead twigs of Camellia sinensis in Sichuan, China. Phytotaxa 716 (3): 189-202, DOI: 10.11646/phytotaxa.716.3.3, URL: https://doi.org/10.11646/phytotaxa.716.3.3
03F62E65F754E82CFF78FA95FAFC4D76.taxon	materials_examined	Material examined: — CHINA, Sichuan Province, Qionglai City, Tiantaishan Town, 30 ° 15 ′ 58 '' N, 103 ° 6 ′ 25 '' E, 1,000 – 1,250 m, on dead twigs of tea (Camellia sinensis), 18 September 2023, M. F. Chi, X. D. Liang, T 03 (HUEST 24.0219; living culture UESTCC 24.0202); ibid., Sichuan Province, Leshan City, Muchuan County, Muxi Town, Jianhe Township, 28 ° 53 ′ 45 '' N, 103 ° 49 ′ 58 '' E, 1,000 – 1,200 m, on dead twigs of tea (Camellia sinensis), 24 October 2023, M. F. Chi, X. D. Liang, LJ 03 (HUEST 24.0220; living culture UESTCC 24.0203); ibid., Sichuan Province, Meishan City, Hongya County, Wawushan Town, Fuxing Village, 29 ° 64 ′ 29 ’’ N, 102 ° 93 ′ 22 ’’ E, 1,000 - 1,500 m, on dead twigs of tea (Camellia sinensis), 17 July 2023, M. F. Chi, X. D. Liang, EM 18 (HUEST 24.0221; living culture UESTCC 24.0204). Notes: — Helminthosporium velutinum is the most widely distributed and frequently recorded species in this genus, which has been documented on a broad range of woody and herbaceous substrates, with over 110 known host records (Farr 2022). Zhu et al. (2016) was the first to report H. velutinum from a freshwater habitat in China, which is an uncommon environment for this species. Currently, H. velutinum has been observed in both freshwater and terrestrial environments in China (Voglmayr et al. 2017, Chen et al. 2022, Tian et al. 2024). Multi-locus analysis of combined ITS, LSU, SSU, rpb 2, and tef 1 - α sequence data indicate that our collection groups with H. velutinum strains (CBS 139923), supported by 99 % ML and 1.00 BPP statistical values. Additionally, our collection (UESTCC 24.0202, UESTCC 24.0203, UESTCC 24.0204) exhibit morphological characteristics similar to the type strain of H. velutinum strains (CBS 139923), with the shape and color of colonies. Colonies on natural substrates are effuse, black, hairy while the conidiophores are simple, straight or flexuous, cylindrical, smooth, brown to dark brown, with well-defined small pores at the apex. Conidia are with blackish-brown to black scars at the base, obclavate to rostrate, smoothwalled, pale golden brown to brown, 6 - 18 distoseptate (Voglmayr et al. 2017). Hence, based on both morphological comparison and phylogenetic analysis, we report our collection as a new host record on Camellia sinensis.	en	Chi, Mei-Feng, Liang, Xiang-Dong, Wu, Na, Dissanayake, Asha J., Liu, Jian-Kui (2025): Helminthosporium camelliae-sinensis sp. nov., a hyphomycete associated with dead twigs of Camellia sinensis in Sichuan, China. Phytotaxa 716 (3): 189-202, DOI: 10.11646/phytotaxa.716.3.3, URL: https://doi.org/10.11646/phytotaxa.716.3.3
