identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
039687EA6F56FFF4B8A3F892FEE32935.text	039687EA6F56FFF4B8A3F892FEE32935.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction	<div><p>DNA extraction and sequencing</p><p>The CTAB rapid plant genome DNA extraction kit DN14 (Aidlab Biotechnologies, Beijing, China) was used to extract DNA from dried samples. The experimental steps referred to the instructions of the kit and the modified methods in Song et al. (2022). The primers used for the amplification of the ITS gene fragment were ITS5 and ITS4 (White et al. 1990), and the Polymerase Chain Reaction (PCR) procedure was as follows: initial denaturation at 95℃ for 3 min, followed by 35 cycles at 94℃ for 40 s, 56℃ for 45 s, and 72℃ for 1 min, and a final extension of 72℃ for 10 min. The primers used for the amplification of the nLSU gene fragment were LR0R and LR7 (Hopple &amp; Vilgalys 1999), and NS1 and NS4 were used for the amplification of the nSSU gene fragment (Hibbett, 1996). The PCR procedure for nLSU and nSSU were as follows: initial denaturation at 94℃ for 1 min, followed by 35 cycles at 94℃ for 30 s, 50℃ for 1 min, and 72℃ for 90 s, and a final extension of 72℃ for 10 min. The primers used for the amplification of the rpb2 gene fragment were 5F and 7Cr (Liu et al. 1999), and the PCR procedure was as follows: initial denaturation at 94℃ for 2 min, 9 cycles at 94℃ for 45 s, 60℃ for 45 s, followed by 36 cycles at 94℃ for 45 s, 53℃ for 1 min, 72℃ for 90 s, and a final extension of 72℃ for 10 min. The PCR amplification products were sequenced at the Beijing Genomics Institute (BGI), China, with the same primers. The newly generated sequences in this study were uploaded to Genbank (the National Center for Biotechnology Information, USA), and all the sequences used in this study were shown in Table 1.</p></div>	https://treatment.plazi.org/id/039687EA6F56FFF4B8A3F892FEE32935	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Fan, Xiang-Ping;Song, Chang-Ge;Xu, Yi-Hua;Sun, Yi-Fei;Cui, Bao-Kai	Fan, Xiang-Ping, Song, Chang-Ge, Xu, Yi-Hua, Sun, Yi-Fei, Cui, Bao-Kai (2025): Morphology and molecular phylogeny reveal one new species of Hydnellum (Thelephorales) from Eastern China. Phytotaxa 711 (1): 43-60, DOI: 10.11646/phytotaxa.711.1.3, URL: https://doi.org/10.11646/phytotaxa.711.1.3
039687EA6F5EFFFFB8A3FF32FC552E0E.text	039687EA6F5EFFFFB8A3FF32FC552E0E.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Hydnellum hangzhouense X. P. Fan, Y. F. Sun & B. K. Cui 2025	<div><p>Hydnellum hangzhouense X.P. Fan, Y.F. Sun &amp; B.K. Cui sp. nov. (Figures 2–4)</p><p>MycoBank no.: MB 857253</p><p>Diagnosis:—Differs from H. crassipileatum and H. chocolatum by its thick pileus, azonate pileal surface with light brown to dark brown color, and relatively narrower basidiospores.</p><p>Type:— CHINA. Zhejiang Province: Hangzhou, Chunan, Hongye Bay Scenic Area, on the ground of the mixed coniferous-broadleaf forest, 29°37′55.308″N, 118°57′35.719″E, elev. 124 m, 15 May 2024, Wu 1679 (Holotype, BJFC045989).</p><p>Etymology:— hangzhouense (Lat.) refers to the collection site of this specimen.</p><p>Description:— Basidiomata annual, eccentrically stipitate, single to concrescent, with odor or taste when fresh. Pileus circular to elliptical, up to 3.8 cm in diam and 0.7 cm thick at the center. Pileal surface light brown (6D5) to dark brown (6F5) when fresh, becoming grayish brown (6E3) upon drying, azonate, tomentose; margin white to grayish brown (6E3) when fresh, becoming dark brown (6F7) upon drying, up to 6 mm wide. Context light brown (6D4) when fresh, dark brown (6F7) upon drying., fragile, up to 5 mm thick. Spines soft, light brown (6D5) to white when fresh, becoming light brown (6D8) to grayish brown (7E4) upon drying, fragile, and up to 6 mm long. Stipe cylindrical and glabrous, surface layer grayish brown (7E4), and inner layer dark brown (6F5), and up to 3 cm long and 1.3 cm in diam.</p><p>Hyphal structure:—Hyphal system monomitic; generative hyphae with simple septa; all the hyphae IKI–, CB–, turned to olive-green in KOH.</p><p>Context:—Generative hyphae colorless to clay-buff, thick-walled, branched, interwoven to regularly arranged, 3.5–5.5 µm in diam.</p><p>Spines:—Generative hyphae colorless to clay-buff, thick-walled, rarely branched, interwoven to regularly arranged, 2.5–4.5 µm in diam. Cystidia and cystidioles absent. Basidia clavate, bearing four sterigmata (3–4.5 µm long) and a basal simple septum, 16–33 × 5–7.5 µm; basidioles similar to basidia in shape, but slightly smaller.</p><p>Stipe:—Generative hyphae colorless to clay-buff, thick-walled, rarely branched, interwoven in the surface layer, regularly arranged in the inner layer, and 2.5–6.5 µm in diam.</p><p>Spores:—Basidiospores subellipsoid to ellipsoid, colorless, thin-walled, echinulate, IKI–, CB–, 4–5.6(–6) × (3–)3.2–5(–5.5) µm, L = 4.85 µm, W = 3.25 µm, Q = 1.49 (n = 90/1, without the ornamentation).</p><p>Ecological habits:— H. hangzhouense was collected from eastern China with subtropical monsoon climate. It grows on the moist ground of the moss and mixed coniferous-broadleaf forest.</p></div>	https://treatment.plazi.org/id/039687EA6F5EFFFFB8A3FF32FC552E0E	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Fan, Xiang-Ping;Song, Chang-Ge;Xu, Yi-Hua;Sun, Yi-Fei;Cui, Bao-Kai	Fan, Xiang-Ping, Song, Chang-Ge, Xu, Yi-Hua, Sun, Yi-Fei, Cui, Bao-Kai (2025): Morphology and molecular phylogeny reveal one new species of Hydnellum (Thelephorales) from Eastern China. Phytotaxa 711 (1): 43-60, DOI: 10.11646/phytotaxa.711.1.3, URL: https://doi.org/10.11646/phytotaxa.711.1.3
