taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
62413E23FFFBFF8B614AFA0CFB0FF8CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/15864085/files/figure.png	https://doi.org/10.5281/zenodo.15864085	FIG. 6. Summaries of diatom OTU responses to (a) total phosphorus and (b) total nitrogen. In panel b, x-axis break is 1,499– 3,999 µg/L. Crossed circles are results directly from TITAN (change points [CP] with bootstrapped 5th and 95th quantiles and mid-points in the portions of cumulative frequency distributions showing the greatest increases in bootstrapped change points). Circles are boosted regression tree (BRT) results showing mid-points from portions of partial dependence plots with substantial change in response variables. Squares are peaks in standardized splits density plots in gradient forest (GF) analysis. Colors denote responses by low nutrient diatom OTUs (blue), high nutrient diatom OTUs (red), and assemblage change (gray; GF and nonmetric multidimensional scaling [NMDS]). Horizontal lines represent the range within which notable responses occurred (or 5th and 95th quantiles of TITAN change points). Diatom responses occurred at multiple points along the TP or TN gradient and these are denoted as BRT1, BRT2..., GF1, GF2..., and sumZ1, sumZ2. Vertical dashed gray lines demarcate portions of TP and TN gradients within which substantial changes in diatom assemblage occurred (see Summary of diatom responses in Results).	FIG. 6. Summaries of diatom OTU responses to (a) total phosphorus and (b) total nitrogen. In panel b, x-axis break is 1,499– 3,999 µg/L. Crossed circles are results directly from TITAN (change points [CP] with bootstrapped 5th and 95th quantiles and mid-points in the portions of cumulative frequency distributions showing the greatest increases in bootstrapped change points). Circles are boosted regression tree (BRT) results showing mid-points from portions of partial dependence plots with substantial change in response variables. Squares are peaks in standardized splits density plots in gradient forest (GF) analysis. Colors denote responses by low nutrient diatom OTUs (blue), high nutrient diatom OTUs (red), and assemblage change (gray; GF and nonmetric multidimensional scaling [NMDS]). Horizontal lines represent the range within which notable responses occurred (or 5th and 95th quantiles of TITAN change points). Diatom responses occurred at multiple points along the TP or TN gradient and these are denoted as BRT1, BRT2..., GF1, GF2..., and sumZ1, sumZ2. Vertical dashed gray lines demarcate portions of TP and TN gradients within which substantial changes in diatom assemblage occurred (see Summary of diatom responses in Results).	2020-12-31	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.		Zenodo	biologists	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.			
62413E23FFFBFF8B614AFA0CFB0FF8CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/15864081/files/figure.png	https://doi.org/10.5281/zenodo.15864081	FIG. 4. Partial dependence plots from boosted regression trees showing responses of nonmetric multidimensional scaling (NMDS) axis 1 scores and of diatom metrics to TP or TN while controlling for the average effect of other variables. FF, fitted functions. Rug plots show deciles of predictor values.	FIG. 4. Partial dependence plots from boosted regression trees showing responses of nonmetric multidimensional scaling (NMDS) axis 1 scores and of diatom metrics to TP or TN while controlling for the average effect of other variables. FF, fitted functions. Rug plots show deciles of predictor values.	2020-12-31	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.		Zenodo	biologists	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.			
62413E23FFFBFF8B614AFA0CFB0FF8CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/15864083/files/figure.png	https://doi.org/10.5281/zenodo.15864083	FIG. 5. Results from gradient forest analysis showing diatom OTUs and assemblage responses to TP and TN. (a) Compositional change based on aggregating OTU responses were determined by split importance and values along TP and TN gradients (bars) and the ratios (blue lines) of split density (black lines) to data density (red lines). Peaks and regions of standardized split density plots with ratios>1 (horizontal dashed lines) delineate portions of the TN or TP gradient within which OTU compositional change is relatively greater than elsewhere along the nutrient gradient. (b) Cumulative change of individual OTUs is shown based on their splits’ importance distributions, which were scaled by R2 and standardized by data density for each OTU with the most important OTUs being labeled. (c) Relative rates of overall change in assemblage composition based on cumulative splits importance plots along the TP and TN gradients.	FIG. 5. Results from gradient forest analysis showing diatom OTUs and assemblage responses to TP and TN. (a) Compositional change based on aggregating OTU responses were determined by split importance and values along TP and TN gradients (bars) and the ratios (blue lines) of split density (black lines) to data density (red lines). Peaks and regions of standardized split density plots with ratios>1 (horizontal dashed lines) delineate portions of the TN or TP gradient within which OTU compositional change is relatively greater than elsewhere along the nutrient gradient. (b) Cumulative change of individual OTUs is shown based on their splits’ importance distributions, which were scaled by R2 and standardized by data density for each OTU with the most important OTUs being labeled. (c) Relative rates of overall change in assemblage composition based on cumulative splits importance plots along the TP and TN gradients.	2020-12-31	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.		Zenodo	biologists	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.			
62413E23FFFBFF8B614AFA0CFB0FF8CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/15864079/files/figure.png	https://doi.org/10.5281/zenodo.15864079	FIG. 2. Nonmetric multidimensional scaling (NMDS) ordination of all samples (n = 342) using relative abundances of OTU rbcL sequences. Spearman correlation overlays show relationships of axes with land cover, nutrients, and diatom metrics (gray lines). Axes are scaled to coincide with the possible range of correlations from —1 to 1. Ag, agriculture; TP, total phosphorus; TN, total nitrogen; HP, LP, HN, and LN, relative abundances of high and low phosphorus and nitrogen diatoms.	FIG. 2. Nonmetric multidimensional scaling (NMDS) ordination of all samples (n = 342) using relative abundances of OTU rbcL sequences. Spearman correlation overlays show relationships of axes with land cover, nutrients, and diatom metrics (gray lines). Axes are scaled to coincide with the possible range of correlations from —1 to 1. Ag, agriculture; TP, total phosphorus; TN, total nitrogen; HP, LP, HN, and LN, relative abundances of high and low phosphorus and nitrogen diatoms.	2020-12-31	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.		Zenodo	biologists	Smucker, Nathan J.;Pilgrim, Erik M.;Nietch, Christopher T.;Darling, John A.;Johnson, Brent R.			
