taxonID	type	description	language	source
BBC8A00DAD3D5B9A88A64100F966A422.taxon	etymology	Etymology. The species kregeriana (kre. ge. ri. a’na. N. L. fem. adv.) is named in honor of the late Dr. Nelly Jeanne Wilhelmina Kreger-van Rij for her work in the field of microbiology, particularly in yeast systematics and ultrastructure.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
BBC8A00DAD3D5B9A88A64100F966A422.taxon	description	Culture characteristics. After growth on YPD agar for 3 days at 25 ° C, colonies are white, butyrous, nearly circular, lightly raised, rough and wrinkled with irregular surfaces and margins (Fig. 3 A). Cells are oval-shaped or oval (3.3 – 4.5 × 4.9 - 8.7 μm) and budding is multilateral (Fig. 3 B). No pseudohyphae are formed. Asci (2.4 – 4.6 × 5.0 – 5.4 um) are persistent and conjugated, typically forming two to four spherical spores within shuttle-shaped or triangular ascospores (Fig. 3 C). The sexual structures were observed on YCB agar after 30 days at 25 ° C. Conjugation can occur between a cell and its bud, or between independent cells.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
BBC8A00DAD3D5B9A88A64100F966A422.taxon	description	Physiological and biochemical characteristics. Glucose is not fermented. Glucose, ethanol, N-acetyl-D-glucosamine, glycerol (slow), D-glucosamine (weak), inulin (weak), sucrose (weak) and succinic acid are assimilated as sole carbon sources. D-galactose, L-sorbose, erythritol, D-xylose, glucitol, D-maltose, sodium citrate dihydrate, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, soluble starch, L-arabinose, D-arabinose, xylitol, D-ribose, L-rhamnose, methanol, ribitol, galactitol, D-mannitol, α-methyl-D-glucoside, salicin, D-glucuronic acid, DL-lactic acid, inositol and hexadecane are not assimilated as sole carbon sources. Ethylamine hydrochloride, cadaverine dihydrochloride, L-lysine and ammonium sulfate are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated as sole nitrogen sources. Growth in vitamin-free medium and on 50 % (w / v) glucose are positive, while growth in 10 % NaCl plus 5 % glucose medium and on 60 % (w / v) glucose are negative. Diazonium blue B, urease activity and production of extracellular starch-like compounds are negative. Growth on YPD agar at 30 ° C is positive, but negative at 37 ° C.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
C6AB4321CD9159DA8D430345A904AC26.taxon	etymology	Etymology. This species phaffii (phaf’fi. i. N. L. gen. n.) is named in honor of the late Prof. Herman Phaff for his significant contributions to the field of microbiology, particularly in yeast systematics and ecology.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
C6AB4321CD9159DA8D430345A904AC26.taxon	description	Culture characteristics. After growth on YPD agar for 3 days at 25 ° C, colonies are white butyrous, rough and wrinkled with irregular surfaces and margins (Fig. 2 A). Cells are ovoid to elongate (2.1 – 3.2 × 3.3 – 7.1 μm) and occur singly or in pairs (Fig. 2 B). Budding is multilateral and pseudohyphae are formed (Fig. 2 D). Asci (2.8 – 3.5 × 6.2 – 7.1 μm) are persistent and unconjugated, typically forming two to four spherical spores within diamond-shaped ascus ascospores, although a linear arrangement of ascospores is seen as well (Fig. 2 C). The sexual structures were observed on YCB agar after 30 days at 25 ° C.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
C6AB4321CD9159DA8D430345A904AC26.taxon	description	Physiological and biochemical characteristics. Glucose is not fermented. Glucose, ethanol, erythritol (weak), succinic acid, sodium citrate dihydrate (weak), D-glucosamine, inulin (weak) and DL-lactic acid are assimilated as sole carbon sources. D-galactose, L-sorbose, D-xylose, glycerol, glucitol, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, soluble starch, N-acetyl-D-glucosamine, L-arabinose, D-arabinose, xylitol, D-ribose, L-rhamnose, methanol, ribitol, galactitol, D-mannitol, α-methyl-D-glucoside, salicin, D-glucuronic acid, citrate acid, inositol and hexadecane are not assimilated as sole carbon sources. Ethylamine hydrochloride, cadaverine dihydrochloride, L-lysine, and ammonium sulfate are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated as sole nitrogen sources. Growth in vitamin-free medium and on 50 % (w / v) glucose are positive, while growth in 10 % NaCl plus 5 % glucose medium and on 60 % (w / v) glucose is negative. Diazonium blue B, urease activity and production of extracellular starch-like compounds are negative. Growth on YPD agar at 30 ° C is positive, but negative at 37 ° C.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
E7AEBDD7D9615210A6F9CF408410D72C.taxon	etymology	Etymology. The specific epithet ureolytica (u. re. o. ly’ti. ca. L. fem. adj.) derived from “ urea ” (the compound that can be broken down) and “ lyticus, ” which indicates the ability to break down or decompose urea.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
E7AEBDD7D9615210A6F9CF408410D72C.taxon	description	Culture characteristics. After growth on YPD agar for 3 days at 25 ° C, colonies are white, circular, butyrous and smooth with entire margins (Fig. 4 A). Cells are ovoid (3.5 – 5.0 × 3.8 – 5.2 μm) and occur singly or in pairs. Budding is multilateral and pseudohyphae are not formed (Fig. 4 B). Asci (4.7 – 5.4 × 5.5 – 6.2 μm) are persistent, typically forming four spherical spores within a diamond-shaped or subrounded ascus. (Fig. 4 C). The sexual structures were observed on YCB agar after 30 days at 25 ° C.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
E7AEBDD7D9615210A6F9CF408410D72C.taxon	description	Physiological and biochemical characteristics. Glucose fermentation is weak. Glucose, ethanol, succinic acid (slow / weak) and DL-lactic acid are assimilated as sole carbon sources. Galactose, L-sorbose, D-xylose, D-glucosamine, glycerol, glucitol, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, N-acetyl-D-glucosamine, L-arabinose, D-arabinose, xylitol, D-ribose, L-rhamnose, methanol, erythritol, ribitol, galactitol, D-mannitol, α-methyl-D-glucoside, salicin, D-glucuronic acid, sodium citrate dihydrate, inositol and hexadecane are not assimilated as sole carbon sources. Ethylamine hydrochloride, cadaverine dihydrochloride, L-lysine and ammonium sulfate are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated as sole nitrogen sources. Growth in vitaminfree medium and on 50 % and 60 % (w / v) glucose are positive, while growth in 10 % NaCl plus 5 % glucose medium is negative. Urease activity is positive (Suppl. material 4). Diazonium blue B reaction and production of extracellular starchlike compounds are negative. Growth on YPD agar at 30 ° C is positive, but negative at 37 ° C.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
719BC3C13C6C5A73BCFB234CA5C4A2ED.taxon	etymology	Etymology. The specific epithet wuzhishanensis (wu. zhi. han’en. sis. N. L. fem. adj.) is named after the location where the type strain of the species was isolated: Wuzhishan city, Hainan province, China.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
719BC3C13C6C5A73BCFB234CA5C4A2ED.taxon	description	Culture characteristics. After growth on YPD agar for 3 days at 25 ° C, colonies are white, butyrous, circular and glossy, with a smooth surface and entire margins (Fig. 5 A). Cells are oval-shaped or oval (2.1 – 4.5 × 3.1 – 5.7 μm) and budding is monopolar (Fig. 5 B). Sexual structures were not observed in single or mixed strain cultures on CMA, PDA and V 8 agar after 2 months of incubation at 25 ° C.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
719BC3C13C6C5A73BCFB234CA5C4A2ED.taxon	description	Physiological and biochemical characteristics. Glucose is not fermented. Glucose, ethanol, N-acetyl-D-glucosamine, glycerol, D-glucosamine, succinic acid and DL-lactic acid (weak) are assimilated as sole carbon sources. D-galactose, L-sorbose, erythritol, D-xylose, glucitol, maltose, sodium citrate dihydrate, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, soluble starch, L-arabinose, D-arabinose, xylitol, D-ribose, L-rhamnose, methanol, ribitol, galactitol, D-mannitol, α-methyl-D-glucoside, salicin, D-glucuronic acid, inositol, sucrose, inulin and hexadecane are not assimilated as sole carbon sources. Ethylamine hydrochloride, cadaverine dihydrochloride, L-lysine and ammonium sulfate are assimilated as sole nitrogen sources. Sodium nitrite and potassium nitrate are not assimilated as sole nitrogen sources. Growth in vitamin-free medium and on 50 % (w / v) glucose are positive, while growth in 10 % NaCl plus 5 % glucose medium and on 60 % (w / v) glucose are negative. Diazonium blue B, urease activity and production of extracellular starch-like compounds are negative. Growth on YPD agar at 37 ° C is positive, but negative at 42 ° C.	en	Guo, Liang-Chen, Hu, Shuang, Zhu, Hai-Yan, Shang, Yu-Jie, Qiu, Yan-Jie, Wen, Zhang, Chen, Shen-Xi, Bai, Feng-Yan, Han, Pei-Jie (2025): Four new species of Pichia (Pichiales, Pichiaceae) isolated from China. MycoKeys 114: 115-132, DOI: 10.3897/mycokeys.114.142474
