Description of Lactobacillus sakei subsp. carnosus M3

Cells are Gram-staining positive, non-flagellated, and rod. Colonies are circular, raised, entire, and white colored after 2 days of incubation on MRS agar at 30℃. Positive for β -glucosidase, L- arabinose in API 20NE, but negative for nitrate reduction, reduction of nitrates to nitrogen, indole production, glucose acidification, arginine dihydrolase, urease, protease, β -galactosidase, D- glucose, D- mannose, D- mannitol, N -acetyl-glucosamine, D- maltose, gluconate, caprate, adipate, malate, citrate, phenyl-acetate. D- melibiose and L- arabinose are utilized. Does not utilize D- mannitol, D- glucose, salicin, L- fucose, D- sorbitol, propionate, caprate, valerate, citrate, L- histidine, 2-ketogluconate, 3-hydroxy-butyrate, 4-hydroxy-benzoate, L- proline, L- rhamnose, N -acetyl-glucosamine, D- ribose, inositol, D- sucrose, D- maltose, itaconate, suberate, malonate, acetate, D, L- lactate, L- alanine, 5-ketogluconate, glycogen, 3-hydroxy-benzoate, and L- serine.

Strain M3 (= NIBRBAC000503070) was isolated from the intestinal tract of a finless porpoise ( Neophocaena phocaenoides), Chungcheongnam Province, Korea.