Description of Flavobacterium cutihirudinis R23R4

Cells are Gram-stain-negative, non-flagellated and rod shaped. Colonies are irregular, raised and yellow colored on R2A agar medium after 48 h incubation at 30℃. Cells reduce nitrate to nitrogen and positive for β ­glucosidase, β ­galactosidase, alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, α ­chymotrypsin, acid phosphatase, naphthol­AS­BI­phosphohydrolase, α ­galactosidase, β ­galactosidase, α ­glucosidase, β ­glucosidase, N ­acetyl­ β ­glucosaminidase, α ­fucosidase (API 20NE/ ZYM). Assimilate D­glucose, L­arabinose, D­mannose, N ­ acetyl­D­glucosamine, D­maltose, malate, D­sucrose, glycogen (API 20NE/ID 32GN) and utilize dextrin, D­maltose, D­cellobiose, gentiobiose, sucrose, D­turanose, stachyose, D­raffinose, α ­D­lactose, D­melibiose, N ­glucosamine, β ­mannosamine, N ­galactosamine, neuraminic acid, α ­D­glucose, D­mannose, D­fructose, D­galactose, 6­methyl­glucose, L­fucose, D­fructose, gelatin, glycyl­L­proline, L­arginine, L­aspartic acid, L­glutamic acid, L­histidine, L­serine, pectin, galacturonic acid, galactonic lactone, D­glucuronic acid, glucuronamide, Tween40, acetoacetic acid and acetic acid as sole carbon sources (Biolog GEN III).

Strain R23 R4 (= NIBRBAC000503079) was isolated from the gut of Korean striped bitterling ( Acheilognathus yamatsutae) at Yeongwol, Gangwon Province, Korea. The DNA G + C content of the type strain is 34 mol %.