Description of Citrobacter portucalensis 765

Cells are Gram­stain­negative, flagellated and coccus shaped. Colonies are circular, convex, entire and beige colored on TSA medium supplemented with 0.5% yeast extract after 48 h incubation at 25℃. Cells reduce nitrate to nitrite and are positive for β ­galactosidase, alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, trypsin, acid phosphatase, naphthol­AS­BI­phosphohydrolase, α ­galactosidase, β ­galactosidase, α ­glucosidase (API 20NE/ZYM). Assimilate D­glucose, L­arabinose, D­mannose, D­mannitol, N ­acetyl­D­glucosamine, D­maltose, gluconate, malate, citrate, phenyl­acetate, D­melibiose, L­fucose, D­sorbitol, propionate, valerate, 2­ketogluconate, 3­hydroxy­butyrate, L­proline, L­rhamnose, D­ribose, inositol, D­sucrose, acetate, lactate, L­alanine, 5­ketogluconate, 3­hydroxy­benzoate, L­serine (API 20NE/ID 32GN) and utilize dextrin, Dmaltose, D­trehalose, D­cellobiose, gentiobiose, sucrose, stachyose, D­raffinose, α ­D­lactose, D­melibiose, β ­Dglucoside, N ­glucosamine, β ­mannosamine, N ­galactosamine, neuraminic acid, α ­D­glucose, D­mannose, D­fructose, D­galactose, 6­methyl­glucose, D­fucose, L­fucose, L­rhamnose, inosine, D­sorbitol, D­mannitol, myo­inositol, glycerol, D­glucose, D­fructose, D­aspartic acid, D­serine, glycyl­L­proline, L­alanine, L­arginine, L­aspartic acid, L­glutamic acid, L­histidine, L­serine, pectin, galacturonic acid, galactonic lactone, D­gluconic acid, D­glucuronic acid, glucuronamide, mucic acid, D­saccharic acid, methyl pyruvate, D­lactic acid, L­lactic acid, citric acid, α ­glutaric acid, D­malic acid, L­malic acid, succinic acid, α ­butyric acid, β ­butyric acid, α ­butyric acid, propionic acid, acetic acid and formic acid as sole carbon sources (Biolog GEN III).

Strain 765 (= NIBRBAC000503088) was isolated from the gut of Leopard mandarin fish ( Siniperca scherzeri) at Chungju, Chungcheongbuk Province, Korea. The DNA G + C content of the type strain is 52 mol %.