Key to adult Dasyhelea of Guadeloupe

(females are unknown for D. brachystyla, huertai, patrycjae, quadrifurca, thomasi and sp. nr. thomsenae)

1 Males ............................................................................................... 2

- Females........................................................................................... 27

2 Aedeagus covered with hyaline envelope (Figs. 1 F, 2E); gonocoxal apodemes and paramere symmetrical (Fig. 2 D) ( cincta group).............................................................................................. 3

- Aedeagus without hyaline envelope (Figs. 4 A, 12A, 22A, 28A, 30A); gonocoxal apodemes and paramere symmetrical or asymmetrical (Figs. 4 C, 12C, 22C, 28C, 30C).............................................................. 4

3 Paramere fused to gonocoxal apodemes; large species, wing length 1.39–1.49 mm .................. D. cincta (Coquillett)

- Paramere not fused to gonocoxal apodemes; small species, wing length 0.98–1.06 mm ...... D. caribbeana Spinelli & Wirth

4 Frontal sclerite broad elliptical, bilobate or heart-shaped (Figs. 5 B, 9A); antennal flagellomere 13 with tapered slender apex (Figs. 3 A, 5B, 7A, 9A); tergite 9 usually with long apicolateral processes (Figs. 4 A, 6A, 10A); gonocoxite often with mesal hook-like, pointed or blunt spur (Figs. 4 A, 6A, 10A) ( grisea group)............................................. 5

- Frontal sclerite slender elliptical (Figs. 11 A, 17B); antennal flagellomere 13 usually without tapered slender apex (Figs. 11 A,19A); tergite 9 with long or short apicolateral processes (Figs. 12 A, 14A, 22A, 28A); gonostylus without mesal hook-like, pointed or blunt spur (Figs. 12 A, 14A, 22A, 30A).......................................................... 15

5 Posterolateral arms of aedeagus with serrate inner-most margins (Figs. 4 D, 8D)................................... 6

- Posterolateral arms of aedeagus with smooth inner-most margins (Figs. 6 D, 10D)................................... 7

6 Tergite 9 with tapered distal portion that extends beyond apices of gonocoxites and elongate tubular, non-divergent apicolateral processes (Fig. 4 A); paramere with slender distal portion with a subapical sharply pointed tubercle and short recurved apex (Fig. 4 C); apices of posterolateral processes of aedeagus abruptly recurved anteroventrally with slender pointed tips (Fig. 4 D)....................................................................................... D. hamula n. sp.

- Tergite 9 with elongate, quadrangular distal portion that extends well beyond apices of gonocoxites and short, triangular, slightly divergent apicolateral processes (Fig. 8 A); paramere with distal portion greatly expanded, flat, triangular, spatula- shaped with recurved tip (Fig. 8 C); apices of posterolateral processes of aedeagus straight with rounded tips (Fig. 8 D)............................................................................................. D. spatula n. sp.

7 Gonocoxite with mesal hook-like, pointed or blunt spur...................................................... 8

- Gonocoxite without mesal hook-like, pointed or blunt spur.................................................. 14

8 Gonocoxite with mesal hook-like spur.................................................................... 9

- Gonocoxite with mesal pointed or blunt spur............................................................... 11

9 Body and genitalia except gonostylus yellowish................................... D. luteogrisea Wirth & Williams

- Body and genitalia brown............................................................................. 10

10 Gonocoxite with large mesal spur; paramere fused to both gonocoxal apodemes................... D. grisea (Coquillett)

- Gonocoxite with small mesal spur; parameres fused to right gonocoxal apodeme.. D. flavifrons (Guérin-Méneville) (in part)

11 Apex of gonostylus with notched tip.......................................... D. pseudoincisurata Waugh & Wirth

- Apex of gonostylus rounded or slightly pointed, not notched................................................. 12

12 Aedeagus with a four-pronged fork-like distal portion (Fig. 6 D)................................ D. quadrifurca n. sp.

- Aedeagus with undivided pair of posterolateral processes (Fig. 10 D)............................................ 13

13 Paramere with broad scalpel-shaped apical portion (Fig. 10 C); tergite 9 apically rounded with short triangular apicolateral pro- cesses (Fig. 10 A); posterior margin of sternite 9 with narrow deep posteromedian excavation (Fig. 10 B)... D. scalpela n. sp.

- Paramere with slender apical portion; tergite 9 apically truncate with elongate tubular apicolateral processes; posterior margin

of sternite 9 with broad, shallow posteromedian excavation................... D. flavifrons (Guérin-Méneville) (in part) 14 Tergite 9 with elongate, tubular apicolateral processes; posterolateral processes of aedeagus without mesally directed arm........................................................................ D. flavifrons (Guérin-Méneville) (in part)

- Tergite 9 with short rounded apicolateral processes; posterolateral processes of aedeagus with mesally directed arm............................................................................................... D. griseola Wirth

15 Frontal sclerite elongate elliptical with lower marginal ear-like lobes (Fig. 15D); gonocoxal apodemes and paramere asymmetrical, paramere elongate, fused to right gonocoxal apodeme (Figs. 12 C, 14C, 16C,18C) ( leptobranchia group).......... 16

- Frontal sclerite elongate elliptical without lower marginal ear-like lobes (Fig. 17 B); gonocoxal apodemes and paramere asymmetrical or symmetrical, fused to both gonocoxal apodemes (Figs. 20 C, 22C, 24C, 26D)............................ 19

16 Palpal segment 3 extremely elongate (Fig. 13 D), palpal ratio 7.13; sternite 9 with posteromedian projection bifid (Fig. 14 B); posterolateral arms of aedeagus stout, sub-triangular (Fig. 14 D).................................. D. patrycjae n. sp.

- Palpal segment 3 short to moderately long, palpal ratio 2.25–4.85; sternite 9 with posteromedian projection entire, not bifid; posterolateral arms of aedeagus slender to stout, but not sub-triangular......................................... 17

17 Posterolateral arms of aedeagus short, stout (Fig. 16 D); gonostylus with distal 1/2 abruptly tapered, slender and greatly curved (Fig. 16 A); posterior margin of sternite 9 greatly elongate, slender (Fig. 16 B)......................... D. waughi n. sp.

- Posterolateral arms of aedeagus elongate, slender; gonostylus with distal 1/2 not abruptly tapered or greatly curved; posterior margin of sternite 9 moderately short, broad............................................................... 18

18 Tergite 9 with greatly divergent apicolateral processes (Fig. 12 A); posterolateral arms of aedeagus broadly separated (Fig. 12 D); distal portion of paramere stout, apex curved 75º (Fig. 12 C)................................. D. juanae n. sp.

- Tergite 9 with parallel apicolateral processes (Fig. 18 A); posterolateral arms of aedeagus narrowly separated (Fig. 18 D); distal portion of paramere slender, apex recurved 180º (Fig. 18 C)....................................... D. thomasi n. sp.

19 Aedeagus with long to moderately long posteromedian projection and posterolateral arms divided or single; gonocoxal apodemes and paramere usually asymmetrical; tergite 9 with well developed conical or elongate cylindrical apicolateral processes ( mutabilis group)................................................................................... 20

- Aedeagus with or without posteromedian projection, posterolateral arms single; gonocoxal apodemes and paramere symmetrical; tergite 9 with very short to moderately elongate apicolateral processes (species unplaced to group)............... 26

20 Gonostylus with deeply bifid apex......................................................... D. scissurae Macfie

- Gonostylus with pointed or rounded apex, not deeply bifid.................................................. 21

21 Gonocoxal apodemes and paramere symmetrical (Figs. 22 C, 24C)............................................. 22

- Gonocoxal apodemes and paramere asymmetrical (Figs. 20 C, 26D)............................................ 23

22 Gonostylus very short, stout with an abruptly tapered apex and sharply pointed tip (Fig. 24 A); paramere moderately broad, the apex extends beyond apex of aedeagus (Fig. 24 A); aedeagus with broad crescent-shaped basal arch, posteromedian projection conical, apex entire (Fig. 24 D); tergite 9 with elongate cylindrical apicolateral processes (Fig. 24 A).... D. brachystyla n. sp.

- Gonostylus long, slender with gradually tapered apex and curved tip (Fig. 22 A); paramere very slender, the apex extends to apex of aedeagus (Figs. 22 A&C); aedeagus with very shallow basal arch, posteromedian projection broad proximally, apex deeply bifurcate; tergite 9 with very short conical apicolateral processes (Fig. 22 A).................. D. megatheca n. sp.

23 Aedeagus moderately short with broadly spaced posterolateral arms............................................ 24

- Aedeagus moderately long with narrowly spaced posterolateral arms............................................ 25

24 Gonostylus bifurcate, short inner gonostylus with subapical prong (Fig. 26 C); aedeagus covered with thin hyaline envelope, posterolateral arms apically divided (Fig. 26 E); posterior margin of sternite 9 with elongate greatly tapered distal portion and slender apex (Fig. 26 B)................................................................... .. D. nelidae n. sp.

- Gonostylus entire, not bifurcate; aedeagus not covered with hyaline envelope with single posterolateral arms; posterior margin of sternite 9 with moderately short non-tapered distal portion with broad apex......... D. guadeloupensis Delécolle & Rieb

25 Aedeagus with tubular posteromedian portion, apex extending to or near apices of gonocoxites, tip flared with recurved apicolateral extensions; tergite 9 with short apicolateral processes; gonocoxal apodemes slightly asymmetrical, barely fused or narrowly separated, paramere small triangular, lightly sclerotized …....................... D. bermudae Wirth & Williams

- Aedeagus with short tubular posteromedian process extending near apices of posterolateral processes, apex narrow, rounded (Fig. 20 D); tergite 9 with elongate apicolateral processes (Fig. 20 A); gonocoxal apodemes asymmetrical, fused to base of paramere, paramere elongate, heavily sclerotized, distal portion J-shaped (Fig. 20 C)............ D. sp. nr. thomsenae Wirth

26 Aedeagus short, broad with short broadly separated posterolateral arms and shallow basal arch (Fig. 30 D); paramere short with truncate apex (Fig. 30 C); posterior extension of sternite 9 narrow with heart-shaped apex (Fig. 30 B); tergite 9 with long apicolateral processes (Fig. 30 A)............................................................... D. turnbowi n. sp.

- Aedeagus elongate with slender long narrowly separated posterolateral arms and deep basal arch (Fig. 28 D); paramere moderately long with arrow-head shaped apex (Fig. 28 C); posterior extension of sternite 9 triangular with rounded apex (Fig. 28 B); tergite 9 with very short apicolateral processes (Fig. 28 A).......................................... D. huertai n. sp.

27 Two spermathecae ( cincta group)........................................................................ 28

- One spermatheca................................................................................... 29

28 Large species, wing length 1.18–1.47 mm; spermathecae small (48 x 42 µm), spherical to ovoid with numerous hyaline punctations; abdominal pleurae with numerous dark streaks........................................ D. cincta (Coquillett)

- Smaller species, wing length 0.99–1.18 mm; spermathecae large (68 x 48 µm), elongate ovoid to sub-quadrangular with sparse hyaline punctations; abdominal pleura without dark streaks........................... D. caribbeana Spinelli & Wirth

29 Frontal sclerite broad elliptical, bilobate or heart-shaped (Figs. 3 F, 7F, 9E) ( grisea group)........................... 30

- Frontal sclerite slender elliptical (Figs. 11 E, 15E, 21F, 25E, 29G).............................................. 37

30 Femora, tibiae with broad dark bands; large species, wing length 1.20–1.47 mm ...... D. pseudoincisurata Waugh & Wirth

- Femora, tibiae without dark bands or with poorly developed band on fore and hind femora; smaller species, wing length 0.84– 1.23 mm ........................................................................................... 31

31 Subgenital plate with arrowhead-shaped anterior portion (Figs. 3 I, 7I, 9H)...................................... 32

- Subgenital plate with slender anterior portion (Figs. 11 H, 15I)................................................ 36

32 Spermatheca with straight to slightly oblique neck (Fig. 3 I).................................................. 33

- Spermatheca with oblique or retort-shaped neck (Figs. 7 I, 9H)................................................ 35

33 Body yellowish............................................................. D. luteogrisea Wirth & Williams

- Body brown to dark brown............................................................................ 34

34 Spermatheca pyriform without distinct neck or very short neck................................ D. grisea (Coquillett)

- Spermatheca ovoid with broad tapered neck (Fig. 3 I)............................................ D. hamula n. sp.

35 Spermatheca with retort-shaped neck (Fig. 9 H)................................................ D. scalpela n. sp.

- Spermatheca with straight or slightly oblique neck................................. D. flavifrons (Guérin-Méneville)

36 Spermatheca spherical with slender oblique neck............................................... D. griseola Wirth

- Spermatheca ovoid with stout elongate, retort-shaped neck (Fig. 7 I)................................. D. spatula n. sp.

37 Frontal sclerite with lower marginal ear-like lobes (Figs. 11 E, 15D) ( leptobranchia group).......................... 38

- Frontal sclerite without lower marginal ear-like lobes (Figs. 21 F, 25E, 29G)...................................... 39

38 Anterior portion of subgenital plate club-shaped, posterior portion elongate, ovoid, with large central lumen; spermatheca large spherical, diameter 86 µm; neck moderately long, slender (Fig. 11 H)............................... D. juanae n. sp.

- Anterior portion of subgenital plate triangular, posterior portion somewhat rectangular without central lumen; spermatheca heavily sclerotized, slightly ovoid, diameter 40 µm, neck short, moderately broad (Fig. 15I)............. D. waughi n. sp.

39 Subgenital plate chalice-shaped (Fig. 29 K) (unplaced to species group)............................. D. turnbowi n. sp.

- Subgenital plate circular to ovoid with a central lumen (Fig. 21 J) ( mutabilis group)............................... 40

40 Spermatheca very large (length 118 µm), elongate ovoid with a moderately long, very slender neck (Fig. 21 I)................................................................................................. D. megatheca n. sp.

- Spermatheca smaller (length 34–71 µm), globular to ovoid with short broad neck................................ 41

41 Spermatheca very small (length 34 µm), ovoid with very short broad neck; wing membrane infuscated; subgenital plate with a circular very lightly sclerotized anterior portion, a slightly rectangular heavily sclerotized posterior portion and a small lumen (Fig. 25 I)............................................................................... D. nelidae n. sp.

- Spermatheca larger (length 44–71 µm), globular or ovoid with short neck; wing membrane not infuscated; subgenital plate cir- cular to ovoidal with a large well defined lumen........................................................... 42

42 Spermatheca spherical with slender retort shaped neck........................... D. guadeloupensis Delécolle & Rieb

- Spermatheca spherical or ovoid with short straight neck..................................................... 43

43 Spermatheca large (length 64–71 µm), globular with moderately broad tubular neck; subgenital plate circular, anterior and pos- terior portions broad with moderately broad lumen.................................. D. bermudae Wirth & Williams

- Spermatheca smaller (length 48 µm), ovoid with short narrow neck; subgenital plate with broad triangular anterior portion, narrow posterior portion and small triangular lumen........................................... D. scissurae Macfie