21. Stigmella calceolarifoliae Diškus & Stonis, 2016

(Figs 1, 19–24, 35)

Stigmella calceolarifoliae Diškus & Stonis, in Stonis et al. 2016d: 79, 84, 85, figs 245–255.

Material examined. 1 ♂ (holotype), 2♀ (paratypes), BOLIVIA, Copacabana (southern shore of Lake Titicaca), 16°09'42"S, 69°05'19"W, elevation 3930 m, mining larvae on Calceolaria sp. 28.iv.2014, ex pupa v.2014, field card no. 5170, A. Diškus, genitalia slide nos AD 650♂ (holotype), AD 649♀, AD 702♀ (paratypes) (ZMUC) .

Diagnosis. Belongs to S. pandora group. The combination of speckled forewing, distally four-lobed uncus, pointed apical processes of valva, long slender lobes of vinculum, transtilla without sublateral processes, and specific set of four large spine-like cornuti distinguishes S. calceolarifoliae from all other Stigmella species. The host-plant Calceolaria (Calceolariaceae) also makes this species distinctive.

Male (Fig. 35). Described in in Stonis et al. 2016d: 84, 85, fig. 246. Forewing length 2.5–2.8 mm; wingspan 5.5–6.2 mm.

Female. Similar to male.

Male genitalia. Illustrated in Stonis et al. 2016d: figs 246–248.

Female genitalia. Illustrated in Stonis et al. 2016d: fig. 249.

Bionomics (Figs 131–133). Larva mines in leaves of Calceolaria (Calceolariaceae) (Figs 131, 132). Larva greyish white; mine in April. Leaf-mine as a gallery strongly widening in distal part (therefore resembling a blotch) (Fig. 133). In the beginning, beige to dark brown frass filling the whole width of the gallery; further the gallery widened to a blotch with dark brown to brown-black frass distributed irregularly, with very wide unfilled areas of the gallery left. Larval exit slit on upper side of the leaf. Cocoon pale beige to beige; length 2.0– 2.2 mm, maximum width 1.0– 1.1 mm.

Distribution (Fig. 1). This species occurs in the Altiplano of the Bolivian Andes (Bolivia: southern shore of Lake Titicaca) at altitudes about 3930 m (Figs 19–24).